Adult Opn4Cre/+;Rosa26CAG-tdTomato-eGFP/+ mice (8–10 weeks) were anesthetized with isoflurane. The virus (Addgene plasmid pAAV-hSyn-Flpo, # 60663, (Xue et al., 2014 (link)), packaged into AAV2 at the NINDS Viral Production Core Facility) was placed on a piece of Parafilm and loaded into a glass microcapillary (Fisher Scientific, Cat. No. 11714) pulled needle (Sutter Instruments, Model P-2000). The glass needle was used to puncture the sclera of the right eye and 1.5μL of virus was injected into the vitreous chamber (Drummond Science Nanoinject II, Cat. No. 3–000-204). After 410 the injection, mice were recovered from anesthesia and placed back into their home cages.
Protocol detail
Inducing Opn4 Expression in Mouse Retina
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Variable analysis
independent variables
- Virus (Addgene plasmid pAAV-hSyn-Flpo, # 60663, packaged into AAV2 at the NINDS Viral Production Core Facility)
- Not explicitly mentioned
- Adult Opn4Cre/+;Rosa26CAG-tdTomato-eGFP/+ mice (8–10 weeks)
- Isoflurane anesthesia
- Injection of 1.5μL of virus into the vitreous chamber of the right eye
- No positive or negative controls were explicitly mentioned.
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