After the primary hippocampal neurons were cultured to the seventh day, the Calcein-AM/PI live cell/dead cell double staining kit (CA1630, Solarbio, Beijing, China) was used to evaluate the inhibitory effect of pioglitazone on neuronal ferroptosis, and the determination was carried out according to the manufacturer’s instructions (Karuppagounder et al., 2018 (link)).
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