Immunoblotting and immunoprecipitation were performed as described previously [50 (link)]. The following antibodies were used for immunoblotting: anti-C/EBP-β (1:1,000; Cell Signaling, Danvers, MA, USA), anti-PRDM16 (1:1,000) [51 (link)], anti-PPARγ (1:1,000; Cell Signaling), anti-Rpb1CTD (RNA polymerase II CTD) (1:1000; Cell Signaling), anti-tubulin (1:3,000; Sigma-Aldrich, St Louis, MO, USA), or anti-β-actin (1:3,000; Sigma).
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