CN2097 was synthesized using N-(9-fluorenyl)methoxycarbonyl (Fmoc)-solid phase peptide synthesis protocols20 (link), as previously described13 (link). CN2097 was purified by RP-HPLC, lyophilized, and exchanged with HCl. Peptide purity was in the range of 90–95% as determined using high-resolution time of flight AXIMA-performance MALDI TOF-TOF mass spectrometer (Shimadzu).
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