Mg-deficient leaf small RNA sequencing

Equal amounts of frozen Mg-deficient or -sufficient leaves from five plants (one plant per pot) were mixed as a biological replicate. Total RNA was extracted from 0.1 g mixed frozen samples using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer's instructions. Mg-deficient and -sufficient leaf sRNA libraries were constructed according to Lu et al. (2014 (link)). Illumina sequencing was performed on a Solexa sequencer at the Beijing Genomics Institute (BGI), Shenzhen, China (Lu et al., 2014 (link)).

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Ma C.L., Qi Y.P., Liang W.W., Yang L.T., Lu Y.B., Guo P., Ye X, & Chen L.S. (2016). MicroRNA Regulatory Mechanisms on Citrus sinensis leaves to Magnesium-Deficiency. Frontiers in Plant Science, 7, 201.

Publication 2016

TRIzol reagent Solexa sequencer

Biological Frozen Leaf Plant Replicate Trizol

Corresponding Organization : Fujian Agriculture and Forestry University

Other organizations : Fujian Academy of Medical Sciences

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Variable analysis

independent variables

Mg-deficient or -sufficient leaves

dependent variables

SRNA libraries

control variables

Equal amounts of frozen Mg-deficient or -sufficient leaves from five plants (one plant per pot) were mixed as a biological replicate
Total RNA was extracted from 0.1 g mixed frozen samples using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer's instructions

controls

No positive or negative controls were explicitly mentioned in the provided information.

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