Xenograft Mouse Model for LSCC

Twenty BALB/c nude mice, 6 weeks old, were provided by Beijing Vital River Laboratory Animal Technology Co. Ltd. All the animals were housed according to a previously described approach (15 (link)). Mice were randomly divided into four groups (n = 5/group): experimental group 1, which was subcutaneously injected with circRNA_103862 downregulated TU212 cells in the dorsal region; experimental group 2, which was subcutaneously injected with circRNA_103862 overexpression AMC-HN-8 cells in the dorsal region; two control groups that were injected with the same amount of TU212 or AMC-HN-8, respectively. The size of the tumor was measured twice a week with calipers, and the volume of xenografts was determined using the formula of a rotational ellipsoid (length × width² × 0.5). Mice were euthanized 3 weeks after cell injection, and the tumor was collected and further analyzed. LSCC cell proliferation was examined using a proliferating cell nuclear antigen (PCNA) in LSCC xenografts.