The native surface of the NPN-O membranes was first deposited with NHS-Diazirine through vacuum phase reaction[45 (link)] and further grafted with PEG1000 (#B22134, Alpha Aesar, MA, USA) largely as described previously.[22 (link)] The PEG reaction temperature (75 °C) was increased for the 5.4 mm × 5.4 mm chips, because it was found that the high temperature increased the solubility of the PEG and helped the reaction on the NPN surface.
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Surface Functionalization of NPN-O Membranes
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Corresponding Organization : University of Rochester
Other organizations : SiMPore (United States), Rochester Institute of Technology
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Variable analysis
independent variables
- Reaction temperature (75 °C)
- Solubility of PEG
- Extent of reaction on NPN surface
- NHS-Diazirine deposition through vacuum phase reaction
- PEG1000 grafting on NPN surface
- Positive control: The PEG reaction was conducted as described previously in reference [22].
- Negative control: Not explicitly mentioned.
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