mIHC was performed using an Opal 7‐color IHC kit (Akoya Biosciences, Marlborough, MA, USA) as previously described [21 (link)]. First, the slices were baked, dewaxed, rehydrated, and then subjected to antigen retrieval in AR6 (Perkin Elmer, pH = 6.0) or EDTA (50×, pH = 9.0, Proandy, Xi'an, Shaanxi, China) antigen repair solution in a microwave oven for 10 min and then allowed to cool to room temperature. Then, the sections were blocked in blocking buffer for 10 min, incubated with primary antibody (Supplementary Table S4) for 1 h, and incubated with a polymer HRP‐conjugated secondary antibody for 10 min. Subsequently, the tissues were stained with the fluorophore‐4 tyramine signal amplification dye. After that, the paraffin sections were again subjected to antigen repair. The aforementioned steps were iteratively repeated until all antigens were labeled. Finally, the slides were stained with DAPI (BD Biosciences, San Jose, CA, USA) for 5 min and mounted with antifade mounting medium (Beyotime, Shanghai, China) after elution. Multispectral imaging and segmentation imaging analysis were performed using a Vectra multispectral imaging system (Perkin‐Elmer, Waltham, MA, USA).