The whole homogenate was obtained from mouse pituitary glands and kidneys and used for immunoblotting analyses as previously described.(9 (link),25 (link)) Protein samples were added to the sample buffer in the presence of 2-mercaptoethanol and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride (Millipore, Billerica, MA) and treated with diluted antibodies. Signals were detected using Immobilon Western (Millipore).
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Immunoblotting Analysis of Pituitary and Kidney Samples
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Other organizations : Tokushima University, Institute of Biomedical Science, Japanese Red Cross Nagoya Daini Hospital, Ryukoku University
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independent variables
- The whole homogenate obtained from mouse pituitary glands and kidneys
- Immunoblotting analyses
- Protein samples were added to the sample buffer in the presence of 2-mercaptoethanol
- Proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
- The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride (Millipore, Billerica, MA)
- Protein samples were treated with diluted antibodies
- Signals were detected using Immobilon Western (Millipore)
- Not explicitly mentioned
- Not explicitly mentioned
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