For chondrogenic differentiation, a previously described protocol was followed [31 (link)]. Briefly, ADSCs were cultured in a normal cell growth medium at 37 °C with 5% CO2 for 24 h, then the cells were cultured with chondrogenic differentiation medium (STEMCELL Technologies), which was replaced every 3 days. After 28–30 days, chondrogenic pellets were fixed with 4% PFA and embedded in Tissue-Tek O.C.T.™ Compound. Chondrogenic pellets were sliced into 7 μm thick sections using a freezing microtome (Leica), and then the sections were stained with 0.1% alcian blue.
Multilineage Differentiation Potential of ADSCs
For chondrogenic differentiation, a previously described protocol was followed [31 (link)]. Briefly, ADSCs were cultured in a normal cell growth medium at 37 °C with 5% CO2 for 24 h, then the cells were cultured with chondrogenic differentiation medium (STEMCELL Technologies), which was replaced every 3 days. After 28–30 days, chondrogenic pellets were fixed with 4% PFA and embedded in Tissue-Tek O.C.T.™ Compound. Chondrogenic pellets were sliced into 7 μm thick sections using a freezing microtome (Leica), and then the sections were stained with 0.1% alcian blue.
Corresponding Organization : Shanghai Sixth People's Hospital
Other organizations : East China University of Science and Technology, State Key Laboratory of Oncogene and Related Genes
Variable analysis
- Adipogenic differentiation medium
- Osteogenic differentiation medium
- Chondrogenic differentiation medium
- Adipogenic differentiation (assessed by Oil Red O staining)
- Osteogenic differentiation (assessed by alizarin red staining)
- Chondrogenic differentiation (assessed by alcian blue staining)
- ADSCs at 90% confluence
- Culture conditions (37 °C, 5% CO2)
- Normal cell growth medium (for chondrogenic differentiation)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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