Histological Analysis of Tissue Samples

The tissues were taken from the fixative solution for paraffin embedding and microtome sectioning. The paraffin sections were stained by hematoxylin-eosin staining kit (Servicebio, Wuhan, China). Briefly, after deparaffinization and rehydration, sections were stained with hematoxylin solution for 5 min followed by being immersed in 1% acid ethanol (1% HCl in 70% ethanol) for 10 s and then rinsed in water for 1 h. Finally, the sections were stained with eosin solution for 3 min and followed by dehydration with graded alcohol and clearing in xylene [14 (link)]. The slides were examined and photographed using an Olympus BX61 fluorescence microscope (Japan). Observed morphological structure of cells. The size of cells (4 random fields of equal area) was analyzed by Image-Pro Plus 6.0 software.