T. gondii RHΔku80Δhxgprt (RHΔku80) and its mutants were maintained in a confluent monolayer of human foreskin fibroblasts (HFFs) as previously described [33 (link)]. To isolate the tachyzoites, the samples were passed through a French press [34 (link)] and 5-μm syringe filter (Millipore, Darmstadt, Germany).
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