The apoptosis assay of A549 and SPCA1 cells was conducted by Annexin V/FITC and PI kit (BD Biosciences, San José, CA). As mentioned previously50 (link), after washing with 0.01 M PBS twice, the cells were stained first with FITC-labeled Annexin-V for 30 min at room temperature and in the dark, and then with PI for 5 min. Finally, the stained cells were immediately tested by flow cytometer (BD Accuri™ C6 Plus). The flow cytometer collected 200,000 to 30,000 cells per test.
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