Cellular signaling pathway regulation

Cycloheximide (CHX), MG132 and quercetin were purchased from Sigma‐Aldrich (USA). quercetin was dissolved in DMSO and then diluted in DMEM in vitro. In in vivo experiments, the solvent for quercetin was 25% hydroxypropyl‐β‐cyclodextrin.
¹⁶ (link),
¹⁷ (link) Anti‐RhoC and SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) were from Abcam (UK). Anti‐NEDD4 E3 ubiquitin protein ligase (NEDD4) and anti‐GAPDH antibodies were obtained from Cell Signaling Technology (USA). The anti‐WW domain containing E3 ubiquitin protein ligase 1 (WWP1) antibody was purchased from Proteintech (China).

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Huang C., Lai W., Mao S., Song D., Zhang J, & Xiao X. (2024). Quercetin‐induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis. Cancer Medicine, 13(4), e7082.

Publication 2024

quercetin anti‐GAPDH

Corresponding Organization :

Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, Guangdong Academy of Medical Sciences, Southern Medical University, Guangdong Provincial People's Hospital

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Variable analysis

independent variables

Cycloheximide (CHX)
MG132
Quercetin

dependent variables

Protein expression of RhoC
Protein expression of SMURF2
Protein expression of NEDD4
Protein expression of WWP1

control variables

GAPDH (used as a loading control)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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