RNA FISH Assay Using Ribo FISH Kit

RNA FISH assay was performed with the Ribo FISH Kit (RiboBio, China) according to the manufacturer’s instructions. The specific procedure was performed in accordance with the manual provided with the kit. Cells were placed on confocal dishes and incubated at 37°C overnight. Cells were then washed and fixed with 4% paraformaldehyde. Paraformaldehyde-fixed cells were washed with PBS and blocked with blocking solution. Cells were hybridized with probes at 37°C overnight. The cells were then observed and photographed under a confocal fluorescence microscope. For the tumor tissue collected, RNA FISH probe labeling and RNA FISH procedures were performed as described previously [21 (link)].