Isolation of Murine Prostate Stromal Cells

For separating prostate stromal cells, dissociated murine prostate cells were prepared as described previously (15 (link)) and stained with Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA), PE-anti Sca-1 antibody (eBioscience, San Diego, CA), Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). Prostate stromal cells are CD31⁻CD45⁻Ter119⁻Sca-1⁺CD49f⁻ (48 (link)). For detecting MDSC cells, dissociated murine prostate cells were stained with Pacific blue-anti CD11b (eBioscience, San Diego, CA), and PE-Cy7-anti Gr-1 antibodies (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR Fortessa and Aria II, respectively (BD Biosciences, San Jose, CA).