Before surgery or any treatment, 5 mL of blood were collected from each patient and placed in an S-Monovette® 9 mL, Serum Gel with Clotting Activator tube (Sarstedt, Nümbrecht, Germany). RNA was extracted from the serum samples using the miRNeasy® Serum/Plasma Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions, as previously described [19 (link)]. RNA was extracted from the stool samples with a Stool Total RNA Purification Kit (Norgen Biotek Corp.), as described by Tarallo et al. [86 (link)].
Pisano A., Griñan-Lison C., Farace C., Fiorito G., Fenu G., Jiménez G., Scognamillo F., Peña-Martin J., Naccarati A., Pröll J., Atzmüller S., Pardini B., Attene F., Ibba G., Solinas M.G., Bernhard D., Marchal J.A, & Madeddu R. (2020). The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer. Cancers, 12(11), 3432.
Corresponding Organization : Instituto de Investigación Biosanitaria de Granada
Other organizations :
Imperial College London, Italian institute for Genomic Medicine, Candiolo Cancer Institute, Austrian Cluster for Tissue Regeneration, Johannes Kepler University of Linz, Austrian Red Cross
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