Transgenic Mouse Strains for Immunology

C57BL/6 mice and the following gene-targeted strains were obtained from Jackson Laboratory (Bar Harbor, ME): C57BL/6-TG(CAG-EGFP), IFNγR1-deficient (B6.129S7-Ifngr1^tm1Agt/J), and a CD45 congenic mouse strain (B6.SJL-Ptprca Pepcb/BoyJ). CD45.1 × CD45.2 mice were generated by crossing C57BL/6 (CD45.2) mice with Pepcb/BoyJ (CD45.1) mice. The MIIG (Mϕ-insensitive to IFNγ) strain was previously described [25 (link)] and a gift of Dr. Michael Jordan. All mice were bred and housed in the Animal Research Facility at Albany Medical College under microisolator conditions.

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McCabe A., Zhang Y., Thai V., Jones M., Jordan M.B, & MacNamara K.C. (2015). Macrophage-lineage cells negatively regulate the hematopoietic stem cell pool in response to IFNγ at steady state and during infection. Stem cells (Dayton, Ohio), 33(7), 2294-2305.

Publication 2015

C57BL/6 mice C57BL/6-TG(CAG-EGFP),

C57bl mice Gene Ifnγ Mice Strain

Corresponding Organization :

Other organizations : Albany Medical Center Hospital, University of Cincinnati Medical Center

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Variable analysis

independent variables

Gene-targeted strains: C57BL/6-TG(CAG-EGFP), IFNγR1-deficient (B6.129S7-Ifngr1tm1Agt/J), and a CD45 congenic mouse strain (B6.SJL-Ptprca Pepcb/BoyJ)
CD45.1 × CD45.2 mice generated by crossing C57BL/6 (CD45.2) mice with Pepcb/BoyJ (CD45.1) mice
The MIIG (Mϕ-insensitive to IFNγ) strain

dependent variables

Measured outcomes not explicitly mentioned

control variables

C57BL/6 mice
All mice were bred and housed in the Animal Research Facility at Albany Medical College under microisolator conditions

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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