Competent P. pastoris GS115 cells were prepared according to the method described by Invitrogen. The plasmid of pPIC9K-Hewl or pPIC9K-XynCDBFV-Hewl was linearized by BglII (TAKARA, Japan), followed by transformation into P. pastoris GS115 by electroporation [19 (link)]. Positive transformants were screened using MD plates without histidine, and high copy transformants were then screened by YPD plates containing 3.5 g/L geneticin G418 [20 ].
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Transformation of P. pastoris GS115 with Hewl
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Corresponding Organization : Chinese Academy of Agricultural Sciences
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Variable analysis
independent variables
- Linearization of the plasmid of pPIC9K-Hewl or pPIC9K-XynCDBFV-Hewl by BglII
- Transformation of the linearized plasmid into P. pastoris GS115 by electroporation
- Positive transformants screened using MD plates without histidine
- High copy transformants screened by YPD plates containing 3.5 g/L geneticin G418
- Competent P. pastoris GS115 cells prepared according to the method described by Invitrogen
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