No cell lines used in this study were found in the database of commonly misidentified cell lines maintained by the ICLAD and NCBI. MCF10A human breast epithelial cells and MDCK II canine kidney epithelial cells were purchased from ATCC and were maintained as previously described11 (link),41 (link). Cell lines were used for no more than twelve passages and were tested periodically for mycoplasma contamination (Lonzo MycoAlert). The cell lines were not authenticated. MDCKII lines expressing control shLuc, shLKB1 clones 11 and 14, and shE-cadherin were generous gifts from Dr. Michael Sebbagh17 (link). MDCKII cells were maintained in DMEM (4g/L D-glucose with L-Glutamine) with 10% FBS (Atlanta Biologicals) and 1× Penicillin/ Streptomycin (Sigma). These lines were chosen for they are both non-tumorigenic epithelial lines that form strong cell-cell adhesions which have been characterized by our laboratory and others11 (link),41 (link)-43 (link). 293GPG cells are a virus-producing cell line that are a derivative of 293T cells and were maintained as described previously11 (link).
Protocol detail
Cell Line Characterization and Maintenance
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MCF10A human breast epithelial cells
MDCK II canine kidney epithelial cells
FBS
Penicillin/ Streptomycin
293t cells
Biologicals
Breast
Cadherin
Canine
Cell adhesions
Cell line
Cells
Clones
Epithelial cells
Gifts
Glucose
Glutamine
Human
Kidney
Mdck cells
Mycoplasma
Penicillin
Streptomycin
Tumorigenic
Virus
Corresponding Organization :
Other organizations : University of Iowa, Inserm, Centre de Recherche en Cancérologie de Marseille, Centre National de la Recherche Scientifique, Aix-Marseille Université
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Variable analysis
independent variables
- Knockdown of LKB1 (shLKB1 clones 11 and 14)
- Knockdown of E-cadherin (shE-cadherin)
- Not explicitly mentioned
- MCF10A human breast epithelial cells
- MDCK II canine kidney epithelial cells
- MDCKII cells expressing control shLuc
- Cell lines were used for no more than twelve passages
- Cell lines were tested periodically for mycoplasma contamination
- MDCKII cells were maintained in DMEM (4g/L D-glucose with L-Glutamine) with 10% FBS and 1× Penicillin/Streptomycin
- 293GPG cells (virus-producing cell line) were maintained as described previously
- Positive control: MDCKII cells expressing control shLuc
- Negative control: Not explicitly mentioned
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