Non-fasting blood samples were collected from each consenting participant immediately after the interview. Samples were processed directly in the study centre within 2 h. After centrifugation, serum and plasma aliquots were prepared for long-term storage at − 80 °C. While the plasma aliquots had been depleted by other analyses in the previous decades, the serum aliquots had remained frozen for 22 years. Only serum aliquots were used in this analysis.
Serum NfL and tau were quantified with commercially available kits on the single molecule array HD-1 analyser (Quanterix, Lexington, MA, USA), as previously described [24 (link), 25 (link)]. Samples were run in duplicate by board-certified technicians blinded to clinical information. The coefficients of variation for all samples reported were < 20%.
Serum NfL and tau were quantified with commercially available kits on the single molecule array HD-1 analyser (Quanterix, Lexington, MA, USA), as previously described [24 (link), 25 (link)]. Samples were run in duplicate by board-certified technicians blinded to clinical information. The coefficients of variation for all samples reported were < 20%.
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