Recombinant Antibody Reagent Generation

The custom recombinant antibody-like MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076; RRID:AB_2665469) (Gibson et al., 2017 (link)). The antibodies used were as follows: PARP-7 (Invitrogen, PA5-40774; RRID:AB_2607074), α-tubulin (Santa Cruz, sc-8035; RRID:AB_628408), β-tubulin (Abcam, ab6046; RRID:AB_2210370), SNRP70 (Abcam, ab51266; RRID:AB_10673827), Flag (Sigma-Aldrich, F3165; RRID:AB_259529), HA (Sigma-Aldrich, H3663; RRID:AB_262051), rabbit IgG fraction (Invitrogen, 10500C; RRID:AB_2532981), goat anti-rabbit HRP-conjugated IgG (Pierce, 31460; RRID:AB_228341), goat anti-mouse HRP-conjugated IgG (Pierce, 31430; RRID:AB_228307), and Alexa Fluor 488 goat anti-mouse IgG (ThermoFisher, A-11001; RRID:AB_2534069).

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Palavalli Parsons L.H., Challa S., Gibson B.A., Nandu T., Stokes M.S., Huang D., Lea J.S, & Kraus W.L. (2021). Identification of PARP-7 substrates reveals a role for MARylation in microtubule control in ovarian cancer cells. eLife, 10, e60481.

Publication 2021

sc-8035 ab6046 F3165 Alexa Fluor 488 goat anti-mouse IgG

Alexa fluor 488 Anti igg Antibodies Antibody Goat Mouse Parp 7 Rabbit Tubulin α tubulin

Corresponding Organization :

Other organizations : The University of Texas Southwestern Medical Center, Union Hospital, Huazhong University of Science and Technology

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Variable analysis

independent variables

Custom recombinant antibody-like MAR binding reagent (anti-MAR)

dependent variables

Not explicitly mentioned

control variables

Rabbit IgG fraction (Invitrogen, 10500C; RRID:AB_2532981)

controls

Positive control: Not explicitly mentioned
Negative control: rabbit IgG fraction (Invitrogen, 10500C; RRID:AB_2532981)

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