Immunohistochemistry Protocol for EMT Markers

IHC studies were performed using the standard streptavidin/peroxidase staining method, as described previously 22 (link). The following antibodies were used: anti-Rac1(1:1000), anti-Snail1(1:200), anti-N-cad (1:500), anti-Twist1(1:1000) (Abcam, Cambridge, UK), anti-E-cad, and anti-Vim (Maxin, Fuzhou, China). All known positive sections served as positive controls. Negative mouse serum and PBS were used instead of the first antibody as a negative control and blank control, respectively.

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Zhou Y., Liao Q., Han Y., Chen J., Liu Z., Ling H., Zhang J., Yang W., Oyang L., Xia L., Wang L., Wang H., Xue L., Wang H, & Hu B. (2016). Rac1 overexpression is correlated with epithelial mesenchymal transition and predicts poor prognosis in non-small cell lung cancer. Journal of Cancer, 7(14), 2100-2109.

Publication 2016

anti-Rac1 anti-Snail1 anti-N-cad anti-Twist1

Antibodies Antibody Mouse Peroxidase Serum Staining method Streptavidin Twist1

Corresponding Organization : Central South University

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Variable analysis

independent variables

Anti-Rac1
Anti-Snail1
Anti-N-cad
Anti-Twist1
Anti-E-cad
Anti-Vim

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

All known positive sections
Negative mouse serum, PBS (used instead of the first antibody)

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