Western Blot Analysis Protocol

Western blot analysis was done as previously described [28 (link)]. The primary and secondary antibodies are described in Table 4 (application WB). Chemiluminescent signal was detected using an ImageQuant LAS 4000 analyser (GE Healthcare) and the quantitative densitometric analysis was done using ImageJ software (U.S. National Institutes of Health).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Korotkov A., Sim N.S., Luinenburg M.J., Anink J.J., van Scheppingen J., Zimmer T.S., Bongaarts A., Broekaart D.W., Mijnsbergen C., Jansen F.E., Van Hecke W., Spliet W.G., van Rijen P.C., Feucht M., Hainfellner J.A., Kršek P., Zamecnik J., Crino P.B., Kotulska K., Lagae L., Jansen A.C., Kwiatkowski D.J., Jozwiak S., Curatolo P., Mühlebner A., Lee J.H., Mills J.D., van Vliet E.A, & Aronica E. (2021). MicroRNA‐34a activation in tuberous sclerosis complex during early brain development may lead to impaired corticogenesis. Neuropathology and Applied Neurobiology, 47(6), 796-811.

Publication 2021

ImageQuant LAS 4000 ImageJ software

Antibodies Densitometric Western blot analysis

Corresponding Organization : Stichting Epilepsie Instellingen Nederland

Other organizations : Korea Advanced Institute of Science and Technology, Netherlands Institute for Neuroscience, University Medical Center Utrecht, Medical University of Vienna, University Hospital in Motol, University of Maryland, Baltimore, Children's Memorial Health Institute, KU Leuven, Universitair Ziekenhuis Brussel, Harvard University, Brigham and Women's Hospital, Medical University of Warsaw, University of Rome Tor Vergata, Epilepsy Research UK, University College London, Epilepsy Society

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Chemiluminescent signal detected using an ImageQuant LAS 4000 analyser (GE Healthcare)
Quantitative densitometric analysis done using ImageJ software (U.S. National Institutes of Health)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided text.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!