Morphological and Dynamic Bone Analysis

Bone slices were placed under an optical or fluorescence microscope, and the morphological and dynamic parameters of the tibial metaphysis were measured within the range of 1–3 mm distal to the epiphyseal plate line using an Opton Contron semi-automatic image processing system (Opton, Germany). The measured morphological parameters included total tissue volume (TTV), sponge bone volume (SBV), trabecular bone volume (TBV), TBV/TTV, trabecular area to volume ratio (S/V), TBV/SBV, mean trabecular plate thickness (MTPT), density (MTPD), and space (MTPS) were measured. The measured dynamic parameters included trabecular osteoid surface (TOS), percentage of tetracycline single- and double-labeled surface to trabecular bone surface (Sfract(s) and Sfract(d)), mean distance between tetracycline double-labeled lines (DDL), mineral appositional rate, and bone formation rate at tissue level (Svf).

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Ma Y., Zhang Y., Lin Y., Ding X, & Zhang Y. (2023). Effects of osteogenic growth peptide C-terminal pentapeptide and its analogue on bone remodeling in an osteoporosis rat model. Open Medicine, 18(1), 20230656.

Publication 2023

Bone Bone bone Bone formation Bone tissue Epiphyseal plate Fluorescence microscope Mineral Mtpd Sponge Tetracycline Tibial Tissue Trabecular bone

Corresponding Organization : Hubei Urban Construction Vocational and Technological College

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total tissue volume (TTV)
Sponge bone volume (SBV)
Trabecular bone volume (TBV)
TBV/TTV
Trabecular area to volume ratio (S/V)
TBV/SBV
Mean trabecular plate thickness (MTPT)
Mean trabecular plate density (MTPD)
Mean trabecular plate space (MTPS)
Trabecular osteoid surface (TOS)
Percentage of tetracycline single-labeled surface to trabecular bone surface (Sfract(s))
Percentage of tetracycline double-labeled surface to trabecular bone surface (Sfract(d))
Mean distance between tetracycline double-labeled lines (DDL)
Mineral appositional rate
Bone formation rate at tissue level (Svf)

control variables

Bone slices were placed under an optical or fluorescence microscope
Morphological and dynamic parameters were measured within the range of 1-3 mm distal to the epiphyseal plate line
Measurements were made using an Opton Contron semi-automatic image processing system (Opton, Germany)

controls

No positive or negative controls were explicitly mentioned in the input text.

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