Endometrial Cancer Cell Cytotoxicity

The half inhibitory concentration (IC50) of endometrial cancer non-stem cells and ECSCs has been previously calculated and found to be 35.81 μg/mL (95%CI = 32.66–39.23 µg/mL) and 63.68 μg/mL (95%CI = 58.34–69.44 µg/mL), respectively. The concentration curve is shown in Figure S1A. Cells were seeded in a 96-well plate with 2 × 10⁴ cells per well. CBP with the IC50 of the endometrial cancer non-stem cells and ECSCs was added to each cell group. After 48 h, CCK8 was added. The absorbance was measured at 450 nm to calculate cell viability.

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Li Q., Kong F., Cong R., Ma J., Wang C, & Ma X. (2023). PVT1/miR-136/Sox2/UPF1 axis regulates the malignant phenotypes of endometrial cancer stem cells. Cell Death & Disease, 14(3), 177.

Publication 2023

Cancer of the endometrial Cancer stem cells Cell Cell viability Endometrial Inhibitory Stem cells

Corresponding Organization :

Other organizations : China Medical University

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Variable analysis

independent variables

Concentration of CBP (half inhibitory concentration (IC50) of endometrial cancer non-stem cells and ECSCs)

dependent variables

Cell viability

control variables

Cell seeding density (2 × 10^4 cells per well)
Incubation time (48 h)
Measurement method (CCK8 assay, absorbance at 450 nm)

controls

Positive control: Endometrial cancer non-stem cells treated with IC50 of CBP
Positive control: ECSCs treated with IC50 of CBP
Negative control: Not explicitly mentioned

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