The bread wheat cultivar, CS, was grown in a greenhouse with controlled conditions of 26°C/14 h light and 20°C/10 h dark. Three different treatments were applied, namely salt stress, cold, and drought stress induced by polyethylene glycol (PEG). During the two-leaf stage, seedlings were treated with Hoagland liquid medium containing 200 mM NaCl for 1, 3, and 6 h (salt stress), 4°C for 1, 3, and 6 h (cold stress), and 20% PEG4000 for 1, 3, and 6 h (drought stress). Seedlings grown in a normal environment without treatment were set as the control. Three biological replicates were set for all the trials.
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