Comprehensive Chromatin Immunoprecipitation Protocol

ASCL1 (#43666), BAP1 (#13271S), H3K27ac (#8173S), H3K4me1 (#5326S), H3K4me3 (#9751), H3K27me3 (#9733), H2AK119Ub (#8240), ASXL2 (#71257), and RING1B (#5694) were purchased from Cell Signaling Technology (CST) company. HSP90 (sc-7947) was purchased from Santa Cruz. Tubulin antibody (E7) was purchased from Developmental Studies Hybridoma Bank. FOXK1 (A301-727A), FOXK2 (A301-729A), and HCFC1 (A301-399A) antibodies were purchased from Bethyl-Laboratories. ASXL1 and ASXL3 antibodies were generated as previously described [11 (link)]. EZH2 inhibitor GSK126 was purchased from Cayman (15415).

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Tsuboyama N., Wang R., Szczepanski A.P., Chen H., Zhao Z., Shi L, & Wang L. (2022). Therapeutic targeting of BAP1/ASXL3 sub-complex in ASCL1-dependent small cell lung cancer. Oncogene, 41(15), 2152-2162.

Publication 2022

ASCL1 H3K27ac H3K4me1 H3K4me3 H3K27me3 H2AK119Ub ASXL2 RING1B HSP90 (sc-7947) Tubulin antibody (E7) GSK126

A301 Antibodies Antibody Cayman Ezh2 Gsk126 H3k4me3 Hsp90 Hybridoma Ring1b Tubulin

Corresponding Organization :

Other organizations : Northwestern University, China Pharmaceutical University

Top 5 similar protocols

Variable analysis

independent variables

ASCL1
H3K27ac
H3K4me1
H3K4me3
H3K27me3
H2AK119Ub
ASXL2
RING1B
HSP90
Tubulin antibody (E7)
FOXK1
FOXK2
HCFC1
ASXL1
ASXL3
EZH2 inhibitor GSK126

dependent variables

Not explicitly mentioned

control variables

Kept constant to ensure valid results

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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