Endovascular Perforation Model for SAH

The endovascular perforation was produced as previously described with modifications8 (link). Anesthesia was induced with sodium pentobarbital (80 mg/kg). After anesthesia of intraperitoneal injection in mice, a laser Doppler flowmetry probe (Moor Instruments Inc., Wilmington, DE) was adhered at the left temporal bone to monitor the blood flow of the left hemispherea. The left common carotid artery (CCA), internal carotid artery (ICA) and external carotid artery (ECA) were exposed and the external carotid artery (ECA) was fashioned into a 3-mm stump. A 6-0 monofilament nylon suture was advanced into the internal carotid artery (ICA) from the ECA stump until resistance was felt, about 1.0 cm from Common carotid artery bifurcation have resistance to insert again after 2 mm pricking blood vessels and then the suture was immediately withdrawn to induce subarachnoid hemorrhage. The cerebral blood flow decreased obviously on doppler blood flow meter monitoring. In the sham group, the procedure is similar, but not pricking blood vessels, blood flow is also no obvious change. We recorded the blood flow changes before and after the process of blood vessel puncture in all animals. The animals were returned to their cages after operation and had free access to food and water.

Free full text: Click here

Li M., Wang W., Mai H., Zhang X., Wang J., Gao Y., Wang Y., Deng G., Gao L., Zhou S., Chen Q, & Wang X. (2016). Methazolamide improves neurological behavior by inhibition of neuron apoptosis in subarachnoid hemorrhage mice. Scientific Reports, 6, 35055.

Publication 2016

laser Doppler flowmetry probe

Anesthesia Animals Blood Blood flow Blood vessel Cerebral blood flow Common carotid artery External carotid artery Felt Flow meter Food Internal carotid artery Intraperitoneal injection Laser doppler flowmetry Mice Nylon Puncture Sodium pentobarbital Stump Subarachnoid hemorrhage Suture Temporal bone

Corresponding Organization :

Other organizations : Wuhan University, Renmin Hospital of Wuhan University, Shenzhen Bao'an District People's Hospital, Brigham and Women's Hospital, Harvard University, Johns Hopkins Medicine, Johns Hopkins University

Top 5 similar protocols

Variable analysis

independent variables

Induction of endovascular perforation as described in the reference

dependent variables

Cerebral blood flow as measured by laser Doppler flowmetry

control variables

Anesthesia (sodium pentobarbital, 80 mg/kg)
Surgical procedures (exposure of left common carotid artery, internal carotid artery, and external carotid artery)
Insertion of 6-0 monofilament nylon suture into internal carotid artery from external carotid artery stump

controls

Sham group: Surgical procedures without pricking blood vessels, no obvious change in blood flow

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!