A panel of seven human pancreatic cancer cell lines (HPCCLs) was used in this study including BxPC-3, Capan-1, FA-6, Panc-1, Mia-Paca-2, Hs766T and CF-PAC-1. All cell lines were cultured routinely at 37°C in a humidified atmosphere (5% CO2) as described previously (15 (link),28 (link)). BxPC-3, Capan-1 and FA-6 were cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA), Panc-1, Mia-Paca-2 and Hs766T were cultured in Dulbecco's modified Eagle's medium (DMEM) (Merck) and CF-PAC-1 was cultured in Iscove's modified medium each supplemented with 10% fetal bovine serum (FBS) (heat inactivated) (Sigma-Aldrich; Merck KGaA), antibiotics penicillin (50 µg/ml), streptomycin (50 µg/ml) and neomycin (50 µg/ml) (Sigma-Aldrich; Merck KGaA). RPMI-1640 and Iscove's modified Dulbecco's medium were supplemented with 2 and 8 mM L-glutamine (Sigma-Aldrich; Merck KGaA) respectively.
Protocol detail
Culturing Pancreatic Cancer Cell Lines
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
RPMI-1640 medium
Dulbecco's modified Eagle's medium (DMEM)
fetal bovine serum (FBS)
penicillin
streptomycin
neomycin
L-glutamine
Antibiotics
Atmosphere
Cancer
Cell lines
Fetal bovine serum
Glutamine
Human
Neomycin
Pancreatic a cell
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : Kingston University, St George's Hospital, University of London, King's College London
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Cell culture conditions: 37°C, 5% CO2, humidified atmosphere
- Cell culture media: RPMI-1640, DMEM, Iscove's modified medium, supplemented with 10% FBS, antibiotics (penicillin, streptomycin, neomycin), and L-glutamine (2-8 mM)
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!