Adora2b-deficient mice,8 (link) mice with tissue-specific Adora2b deletion (for more details see Supplementary Materials ), matched genetic controls, or C57BL/6 mice were used in DSS studies (3–4.5%), as described previously.13 (link),44 (link),45 (link) Adora2b agonist (BAY 60-6583; Tocris Bioscience, Bristol, UK) or vehicle (30% SolutolHS15 in 0.9% saline; BASF, Florham Park, NJ) was administered by subcutaneous osmotic pump (Alzet; DURECT Corporation, Cupertino, CA) at 1.2–1.25 mg kg−1 mouse per day.
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Adora2b Deficiency and DSS-Induced Colitis
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0 9 saline
Adora2b
Bay 60 6583
C57bl mice
Deletion
Genetic controls
Mouse
Osmotic
Tissue specific
Corresponding Organization : University of Colorado Anschutz Medical Campus
Other organizations : Children's Hospital Colorado, University of Colorado Denver
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Variable analysis
independent variables
- Adora2b-deficient mice
- Mice with tissue-specific Adora2b deletion
- Adora2b agonist (BAY 60-6583)
- Vehicle (30% SolutolHS15 in 0.9% saline)
- Outcomes in DSS studies (3–4.5%)
- Matched genetic controls
- C57BL/6 mice
- Matched genetic controls
- C57BL/6 mice
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