Total lung collagen was measured in lung homogenates using the sircol assay (Biocolor Life Science Assays, County Antrim, UK) as per manufacturer's instructions. Peri-bronchiolar collagen was quantified on sections stained with Sirius red and a polarizing lens, with computer-aided image analysis (Leica Microsystems, Milton Keynes, UK) as previously described (30 (link)).
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