Genome-wide gene expression of C. albicans was analysed with C. albicans-specific microarrays (ClinEuroDiag). The red channel represents hybridization with Cy5-labeled C. albicans RNA from experimental mouse sepsis while the green channel always shows hybridization with Cy3-labeled RNA from C. albicans yeast cells growing logarithmically in standard YPD medium at 37 °C with shaking (common control). C. albicans RNA labelling, microarray hybridization, and scanning were performed as previously described24 (link).
Genome-wide gene expression profiling of mouse samples was performed using the MouseRef-8 (link) Expression and MouseWG6 v2.0 BeadChips (Illumina) according to the manufacturer’s instruction. From the RNA isolation, 200 ng total RNA with a Bioanalyzer RIN greater than seven were used for amplification prior to chip hybridization. Samples were analysed using the iScan platform (Illumina) measuring the variation of expression rate of > 42,000 transcripts. All microarray data are MIAME compliant and raw data have been deposited at GEO (GSE83682). The gene expression of liver, spleen, and kidney tissue 8 h, 12 h, 24 h, and 72 h after intravenous infection was compared to control samples of mock infected mice 24 h after PBS injection.
Genome-wide gene expression profiling of mouse samples was performed using the MouseRef-8 (link) Expression and MouseWG6 v2.0 BeadChips (Illumina) according to the manufacturer’s instruction. From the RNA isolation, 200 ng total RNA with a Bioanalyzer RIN greater than seven were used for amplification prior to chip hybridization. Samples were analysed using the iScan platform (Illumina) measuring the variation of expression rate of > 42,000 transcripts. All microarray data are MIAME compliant and raw data have been deposited at GEO (GSE83682). The gene expression of liver, spleen, and kidney tissue 8 h, 12 h, 24 h, and 72 h after intravenous infection was compared to control samples of mock infected mice 24 h after PBS injection.
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