GC-MS Analysis of Volatile Compounds

A GC-MS analysis was performed by a previously reported method with slight modifications [65 (link)]. Briefly, two μL aliquot of freeze-dried and concentrated BRARP extract in chloroform and methanol was analyzed by an Agilent GC-MS 7890A, 5975C (Agilent, USA) to identify the volatile compounds. The column used was HP DB-5 capillary column (30×0.25 mm×0.25 μm; Agilent Technologies). GC oven initial temperature was 50°C for 2 min and programmed to 280°C at a rate of 5°C.min^-1, and finally held at 280°C for 2 min. Operating conditions for GC as follows: hydrogen was used as carrier gas (5 mL.min^-1); the temperature of injector and detector was 250°C and 280°C, respectively; the volume injected was 2 μL in split mode (10: 1). The mass spectra were performed at 70 eV of the mass range of 50∼400.