Culturing and Characterizing IPEC-J2 Cells

The nontransformed porcine intestinal epithelial cell line IPEC-J2, originally isolated from jejunal epithelia of a neonatal unsuckled piglet [17 (link)], was a kind gift of Dr. Jody Gookin, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. IPEC-J2 cells were maintained on six-well Transwell polyester membrane inserts (Corning Inc., Corning, NY, USA) as it was described previously [18 (link)]. Transepithelial electrical resistance (TEER) measurement of monolayers was performed on alternate days after seeding, from day 5 to 21 of culture, using an EVOM Epithelial Tissue Volt/Ohmmeter (World Precision Instruments, Berlin, Germany).

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Palócz O., Szita G, & Csikó G. (2019). Alteration in Inflammatory Responses and Cytochrome P450 Expression of Porcine Jejunal Cells by Drinking Water Supplements. Mediators of Inflammation, 2019, 5420381.

Publication 2019

Transwell polyester membrane inserts EVOM Epithelial Tissue Volt/Ohmmeter

Cell line Cells Electrical resistance Epithelial cell Epithelial tissue Intestinal Jejunal Membrane Neonatal Polyester Porcine

Corresponding Organization : University of Veterinary Medicine

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Variable analysis

independent variables

Time of TEER measurement (from day 5 to 21 of culture)

dependent variables

Transepithelial electrical resistance (TEER) of IPEC-J2 cell monolayers

control variables

IPEC-J2 cell line
Six-well Transwell polyester membrane inserts
EVOM Epithelial Tissue Volt/Ohmmeter

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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