Oral and Lung Cell Culture Protocols

Oral epithelial cells PCS-200-014 (Primary gingival keratinocytes (PGK; ATCC^® PCS-200-014™) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). Lung Fibroblast cells WI-38 were obtained from VACSERA Tissue Culture Unit, Cairo, Egypt. The cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum, 1% L-glutamine, HEPES buffer, and 50 µg/mL gentamycin. All cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂ [44 (link)].

Free full text: Click here

Ismail A.M., Elshewy E.S., El-Ganainy S.M., Magistà D., Hamouda A.F., Alhudaib K.A., Ebrahim W, & Almaghasla M.I. (2023). Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats. Journal of Fungi, 9(3), 282.

Publication 2023

PCS-200-014 ATCC® PCS-200-014™)

Atmosphere Buffer Cells Eagle Epithelial cells Fetal bovine serum Fibroblast cells Gentamycin Gingival Glutamine Hepes Keratinocytes Lung Tissue

Corresponding Organization : Mansoura University

Other organizations : University of Bari Aldo Moro, National Research Council, Institute of Sciences of Food Production, Benha University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Cell lines: Oral epithelial cells PCS-200-014 (Primary gingival keratinocytes) and Lung Fibroblast cells WI-38
Cell culture medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum, 1% L-glutamine, HEPES buffer, and 50 µg/mL gentamycin
Cell culture conditions: 37 °C in a humidified atmosphere with 5% CO2

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!