Fatty acid oxidation in the interscapular BAT from WT and GPRKO female mice were assessed by measuring and summing 14CO2 production and 14C-labeled acid-soluble metabolites from the oxidation of [1-14C] palmitic acid from Perkin Elmer (Waltham, MA) as previously described (24 (link)). Both WT and GPRKO female mice used for this study were fed a HFD for one month starting 12 weeks of age.
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