Eight-day-old rats were injected intraperitoneally with 1 mg/kg PAM3CSK4 (P3C, Merck Millipore France), or 0.9% saline. CSF was sampled 14 h later [5 (link)], and total leukocytes and polymorphonuclear neutrophiles (PMNs) were counted in a Bürker chamber after staining with Türk’s solution (Sigma-Aldrich). The phenotypic analysis of whole blood immune cells was performed by immunocytochemistry following red blood cell lysis as previously described [26 ].
Blondel S., Strazielle N., Amara A., Guy R., Bain C., Rose A., Guibaud L., Tiribelli C., Gazzin S, & Ghersi-Egea J.F. (2022). Vascular network expansion, integrity of blood–brain interfaces, and cerebrospinal fluid cytokine concentration during postnatal development in the normal and jaundiced rat. Fluids and Barriers of the CNS, 19, 47.
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