The ALM and FFM both were assessed in a semi-fasted state by the Tanita MC-780MA (2015, Tanita Corporation, Japan). This Tanita MC-780MA is an 8 electrode multi-frequency (5 kHz/50 kHz/250 kHz) segmental body composition analyzer that predicts ALM and FFM from resistance and reactance (23 (link)). Some research is available on the validity of the device in healthy subjects and patients (34 (link), 35 (link)). Impedance measurement includes whole body and limb segments, working with a constant current source (∼90 A) with a high frequency (50 kHz). Anthropometry data concerning the subject (age, gender, and height) were entered by the accessor in the GMon Health Monitor software. By mounting the BIA scale, body weight was measured, and by holding the two handles with arms separated from the trunk, body composition analysis was performed in 1 min. All BIA measurements followed standard operating procedures. ALM-index (ALMi) and FFM-index (FFMi), our secondary parameters besides FFM, were calculated by dividing ALM and FFM by height in meters squared.
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