Immunofluorescence Analysis of Meiotic Proteins

Immunofluorescence staining was performed as described in the previous studies (Yoon et al., 2018 (link)). The primary antibodies used in this study included as follows: mouse anti-SCP3 (1:600, ab97672, Abcam), rabbit anti-SCP1 (1:600, ab15090, Abcam), rabbit anti-γH2AX (1:300, ab26350, Abcam), and rabbit anti-MLH1 (1:200 ab92312, Abcam). The secondary antibodies used were goat anti-rabbit Alexa 488/594 (1:300, ab150077/ab150080, Abcam) and goat anti-mouse Alexa 488/594 (1:300, ab150113/ab150116, Abcam). Nuclear DNA was stained by 4′,6-diamino-2-phenylindole (DAPI). Images were captured by the fluorescence microscopy Imager M2 (ZEISS, Gottingen, Germany) at 100 × oil objective magnification and an Axiocam 503 monochrome camera.