Western blotting analysis of TSPO, iNOS, and arginase-1

Western blotting analyses were performed as described previously (Wu et al., 2016 (link); Zhou et al., 2017 (link)). The following antibodies were used: rabbit monoclonal anti-TSPO (ab109497, Abcam, Cambridge, MA, USA), rabbit monoclonal anti-iNOS (D6B6S, Cell Signaling Technology, Cambridge, MA, USA), mouse monoclonal anti-β-actin (66009-1-Ig, Proteintech, Wuhan, China), mouse monoclonal anti-β-tubulin (CW0098M, CWBiotech, Beijing, China), and rabbit monoclonal anti-arginase-1 (93668S, Cell Signaling Technology).

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Yao R., Pan R., Shang C., Li X., Cheng J., Xu J, & Li Y. (2020). Translocator Protein 18 kDa (TSPO) Deficiency Inhibits Microglial Activation and Impairs Mitochondrial Function. Frontiers in Pharmacology, 11, 986.

Publication 2020

D6B6S, CW0098M,

Actin Antibodies Arginase 1 Inos Mouse Rabbit Tspo Tubulin Western blotting analyses

Corresponding Organization : Minzu University of China

Other organizations : Institute of Biophysics, Chinese Academy of Sciences, Academy of Military Medical Sciences, Chinese Institute for Brain Research

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of the following proteins: TSPO, iNOS, β-actin, β-tubulin, and arginase-1

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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