THP-1 cells were cultured in RPMI 1640 culture medium (Invitrogen) supplemented with 10% Fetal Bovine Serum (FBS, Corning), 2 mmol/L of glutamine (Gibco) and the antibiotics 100 U/mL penicillin and 100 µg/mL streptomycin (Pen Strep, Gibco), based on a previously described protocol20 (link),61 (link). Briefly, cells were seeded and differentiated using 85 nM Phorbol 12-Myristate 13-Acetate (PMA, Sigma) for 3 days, media was replaced, and cells were allowed to rest for 5 additional days. The medium was replaced and cells were subjected to treatments with 100 µg/mL of the peptides 65-79*LE, 65-79*SE or 65-79*PE with or without recombinant human IFN-γ (10 ng/mL).
RAW 264.7 cells were cultured in DMEM culture medium (Gibco) supplemented with 10% FBS (Corning) and 100 U/mL penicillin and 100 µg/mL streptomycin (Pen Strep, Gibco). Cells were plated and treated with 65-79*LE, 65-79*SE or 65-79*PE (100 µg/mL) with or without recombinant mouse IFN-γ (5 ng/mL).
RAW 264.7 cells were cultured in DMEM culture medium (Gibco) supplemented with 10% FBS (Corning) and 100 U/mL penicillin and 100 µg/mL streptomycin (Pen Strep, Gibco). Cells were plated and treated with 65-79*LE, 65-79*SE or 65-79*PE (100 µg/mL) with or without recombinant mouse IFN-γ (5 ng/mL).
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