Blood serum samples were collected from BLSA participants (AD = 26, CN = 20) at time of AD diagnosis, 43 of whom were included in the autopsy sample, at the NIA Clinical Research Unit in Harbor Hospital, Baltimore. Details on collection and processing have been published previously55 (link). Briefly, venous blood samples were collected between 6 and 7 a.m. following an overnight fast. Serum samples were aliquoted into 0.5-mL volume in Nunc cryogenic tubes and stored at −80 °C until further use. Samples were not subject to any freeze–thaw cycles prior to proteomic assays. Additional details on sample selection have been published previously55 (link). ROS participants (AD = 29, CN = 22) provided blood serum at the time of AD diagnosis, all of whom were included in the autopsy sample, as described previously56 .
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