Immunostainings were carried out using 4 μm tissue sections according to standard procedures. Briefly, after antigen retrieval, samples were blocked with Peroxidase-Blocking Solution (Dako, S202386) for 10 min at RT. Primary antibodies (see Supplementary Table 2) were incubated o/n at 4oC. Slides were washed with EnVision™ FLEX Wash Buffer (Dako, K800721). Corresponding secondary antibody was incubated with the sample for 45 min at RT. Samples were developed using 3,3′-diaminobenzidine, counterstained with hematoxylin and mounted. Staining analyses were performed with QuPath software v.0.3.274 (link) and by histological scoring (H-Score)75 (link).
Rivas E.I., Linares J., Zwick M., Gómez-Llonin A., Guiu M., Labernadie A., Badia-Ramentol J., Lladó A., Bardia L., Pérez-Núñez I., Martínez-Ciarpaglini C., Tarazona N., Sallent-Aragay A., Garrido M., Celià-Terrassa T., Burgués O., Gomis R.R., Albanell J, & Calon A. (2022). Targeted immunotherapy against distinct cancer-associated fibroblasts overcomes treatment resistance in refractory HER2+ breast tumors. Nature Communications, 13, 5310.
Other organizations :
Hospital Del Mar, Hospital del Mar Research Institute, Institute for Research in Biomedicine, Barcelona Institute for Science and Technology, Institute for Bioengineering of Catalonia, INCLIVA Health Research Institute, Universitat de València
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