We utilized our previously described pCI construct for all transient transfections12 (link). A gene encoding MR1B_RFP or MR1C_RFP was ligated into this construct using EcoRI and KpnI, to create pCI_MR1B_RFP or pCI_MR1C_RFP. Confirmatory sequencing was performed. Restriction enzymes and ligation kit were obtained from New England Biolabs (Ipswich, USA). PCR and gel purification kits were obtained from Qiagen (Venlo, Netherlands).
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