IHC staining of the PCa Donor Rapid Autopsy Program at the University of Washington (UWRA, Seattle, WA) metastatic CRPC tissue microarray was performed using SR-B1 primary antibody: AB52629 (Abcam, Cambridge, United Kingdom) (27 (link)). Metastatic specimens were obtained from patients who died of metastatic CRPC, who signed written informed consent for a rapid autopsy performed within 6 h of death under the aegis of the PCa Donor Program at the University of Washington with Institutional Review Board approval. SR-B1 staining was scored by experienced independent pathologists (0 = no staining, 1 = low staining, 2 = moderate staining, 3 = high staining). Expression data for cholesterol metabolism gene transcripts was obtained from 27 patients with paired normal prostatic, and local cancerous, tissue from the Shanghai Changhai Hospital and Fudan University Shanghai Cancer Center (Shanghai Cohort, SC) (28 ) and from 83 CRPC patients from the UWRA; an expansion of the 63 CRPC patient data previously reported (27 (link)).