For western blots, anti-GFP mouse monoclonal (a mixture of clones 7.1 and 13.1, Roche Diagnostics, Meylan, France) was used at 1/1000; rabbit anti-Rod (Scaerou et al. 2001 (link)) was used at 1/6000; rabbit anti-Zw10 (Williams et al. 1992 (link)) was used at 1/1000; and mouse polyclonal anti-Mad1 (Emre et al. 2011 (link)) was used at 1/500. Secondary antibodies, horseradish peroxidase (HRP) anti-mouse (Sigma Chemical Co., Saint-Quentin Fallavier, France) and HRP anti-rabbit (Promega, Charbonnieres, France) were used as described by the suppliers. Western blots were performed as described in (Rubin et al. 2014 (link)).
Protocol detail
Western Blot Antibody Validation
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Corresponding Organization :
Other organizations : Sorbonne Paris Cité, Délégation Paris 7, Université Paris Cité, Centre National de la Recherche Scientifique, Institut Jacques Monod, Cornell University
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Variable analysis
independent variables
- Anti-GFP mouse monoclonal (a mixture of clones 7.1 and 13.1, Roche Diagnostics, Meylan, France)
- Rabbit anti-Rod (Scaerou et al. 2001)
- Rabbit anti-Zw10 (Williams et al. 1992)
- Mouse polyclonal anti-Mad1 (Emre et al. 2011)
- Horseradish peroxidase (HRP) anti-mouse (Sigma Chemical Co., Saint-Quentin Fallavier, France)
- HRP anti-rabbit (Promega, Charbonnieres, France)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: not mentioned
- Negative control: not mentioned
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