Quantitative Immunofluorescence Imaging Analysis

Immunofluorescence and confocal microscope observation were performed, as previously described [6 (link)]. Images were acquired using a TCS SP8 confocal laser scanning fluorescence microscope (Leica, Wetzlar, Germany) equipped with an objective lens (HC PL APO 63x/1.40 OIL CS2, Leica). The number of fluorescent punctae was determined using ImageJ based on the size threshold specified in each legend. For the analysis of green fluorescent protein (GFP)-TFEB, the fluorescence intensities of certain areas in cytoplasmic and nuclear regions were measured using ImageJ.

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Ikari S., Yang Q., Lu S.L., Liu Y., Hao F., Tong G., Lu S, & Noda T. (2021). Quercetin in Tartary Buckwheat Induces Autophagy against Protein Aggregations. Antioxidants, 10(8), 1217.

Publication 2021

HC PL APO 63x/1.40 OIL CS2

Confocal laser scanning microscope Cytoplasmic Fluorescence Green fluorescent protein Immunofluorescence Lens Tfeb

Corresponding Organization : Osaka University

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Variable analysis

independent variables

Immunofluorescence and confocal microscope observation

dependent variables

Number of fluorescent punctae
Fluorescence intensities of certain areas in cytoplasmic and nuclear regions for GFP-TFEB

control variables

Objective lens (HC PL APO 63x/1.40 OIL CS2, Leica)
TCS SP8 confocal laser scanning fluorescence microscope (Leica, Wetzlar, Germany)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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