Protein Expression Analysis by Western Blot

An equal amount of total protein was run on 12.5% SDS-PAGE, transferred to PVDF membranes (250 mA for 2 h), and probed with primary antibodies. The primary antibodies include anti-MMP2 (1:1000, 72 kDa, Sigma-Aldrich), anti-N-cadherin (1:1000, 140 kDa, ImmunoWay Biotechnology), anti-E-cadherin (1:1000, 135 kDa, ImmunoWay Biotechnology), anti-Vimentin (1:1000, 57 kDa, ImmunoWay Biotechnology), anti-ADAM9 (1:1000, 72 kDa, Affinity Biosciences), anti-Snail (1:1000, 29 kDa, ImmunoWay Biotechnology), anti-DNMT1 (1:1000, 183 kDa, Abcam), anti-CD47 (1:1000, 52 kDa, Abcam), and anti-GAPDH (1:1000, 37 kDa, Sigma-Aldrich). The protein bands of interest were captured after the secondary antibodies linked with peroxidase were bound to primary antibodies [26 (link)].