PKCα-overexpressing transgenic (C57), PKCα knockout mice (FVB), and corresponding littermate control hearts were obtained from 12 month old animals (25 (link)). Hearts were homogenized in 1% Triton-100/RIPA buffer containing 1%(v/v) Triton X-100, 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 10 mM EDTA, 10 mM EGTA, phosphatase inhibitor cocktail and protease inhibitors (complete mini-tablet, calpain I and II inhibitors, Roche).
Protocol detail
Cardiac PKCα Overexpression and Knockout
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Animals
Buffer
Calpain i
Edta
Egta
Hearts
Inhibitors
Knockout mice
Nacl
Phosphatase
Pkcα
Protease inhibitors
Ripa
Tablet
Transgenic
Tris
Triton x 100
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Variable analysis
independent variables
- PKCα-overexpressing transgenic (C57)
- PKCα knockout mice (FVB)
- Not explicitly mentioned
- Corresponding littermate control hearts
- Hearts were obtained from 12 month old animals
- Corresponding littermate control hearts
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